Hydroxyl radical scavenging, bnl not radical producing effect of bleomycin
نویسندگان
چکیده
منابع مشابه
Daily hydroxyl radical scavenging capacity of mammals.
Both the formation and reactions of hydroxyl radical (•OH) are quantitative chemical reactions even in mammalians, and so we can reproduce such in vivo reactions in test tubes. Daily urinary excretions of some reaction products have been used to estimate the amount of •OH produced daily. Although urinary 8-hydroxydeoxyguanosine (8-OHdG) is a well-known marker of •OH, we have shown that creatol ...
متن کاملSerum Hydroxyl Radical Scavenging Capacity as Quantified with Iron-Free Hydroxyl Radical Source
We have developed a simple ESR spin trapping based method for hydroxyl (OH) radical scavenging-capacity determination, using iron-free OH radical source. Instead of the widely used Fenton reaction, a short (typically 5 seconds) in situ UV-photolysis of a dilute hydrogen peroxide aqueous solution was employed to generate reproducible amounts of OH radicals. ESR spin trapping was applied to quant...
متن کاملThe production of hydroxyl radical by bleomycin and iron (ii).
Bleomycin 1s a glycopeptidlc antlblotlc used for the treatment of cancer [l] . In vitro, a major effect of bleomycm 1s the mtroduction of strand breaks into DNA [2,3]. Efficient breakage of DNA by bleomycm requues the presence of reducing agents, such as 2-mercaptoethanol, dltiothreitol, ascorbate, or hydrogen peroxlde [3,4]. Although reducing agents alone are injurious to DNA [5,6], the combin...
متن کاملNovel fluorometric assay for hydroxyl radical scavenging capacity (HOSC) estimation.
A novel fluorometric method was developed and validated for hydroxyl radical scavenging capacity (HOSC) estimation using fluorescein as the probe. A constant flux of pure hydroxyl radical is generated under physiological pH using a Fenton-like Fe3+/H2O2 reaction. The generation of pure hydroxyl radicals under the experimental conditions was evaluated and confirmed using electron spin resonance ...
متن کاملMolecular basis of scavenging effect of zonisamide on hydroxyl radical in vitro
Zonisamide (ZNS), a commonly used anticonvulsant, scavenged hydroxyl radicals at a clinically relevant concentration. Reactants of ZNS with hydrogen peroxide and with hydrogen peroxide plus UV irradiation, yielding hydroxyl radicals, were analyzed by the LC/MS technique. Many small fragments were found in the both reactions, suggesting that ZNS was decomposed not only by hydroxyl radicals but a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1997
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)44925-1